Probing the structure of the SARS coronavirus using scanning electron microscopy.
Identifieur interne : 005189 ( Main/Exploration ); précédent : 005188; suivant : 005190Probing the structure of the SARS coronavirus using scanning electron microscopy.
Auteurs : Yun Lin [République populaire de Chine] ; Xiyun Yan ; Wuchun Cao ; Chooying Wang ; Jing Feng ; Jinzhu Duan ; Sishen XieSource :
- Antiviral therapy [ 1359-6535 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- virologie : Syndrome respiratoire aigu sévère.
- instrumentation : Humains, Microscopie électronique à balayage, Virion, Virus du SRAS.
English descriptors
- KwdEn :
- MESH :
- instrumentation : Microscopy, Electron, Scanning.
- methods : Microscopy, Electron, Scanning.
- ultrastructure : SARS Virus, Virion.
- virology : Severe Acute Respiratory Syndrome.
- Humans.
Abstract
A novel coronavirus, SARS-CoV, has been confirmed to be the aetiological agent of SARS. Transmission electron microscope (TEM) images played an important role in initial identification of the pathogen. In order to obtain greater morphological detail of SARS-CoV than could be obtained by TEM, we used ultra-high resolution scanning electron microscopy (SEM) to image the virus particles. We show here the three-dimensional appearance of SARS-CoV. Enhanced detail of the ultrastructure reveals the trimeric structure of the 10-20 nm spikes on the virion surface. These results contribute to characterization of the SARS agent and development of new antiviral strategies.
PubMed: 15134191
Affiliations:
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Chooying" sort="Wang, Chooying" uniqKey="Wang C" first="Chooying" last="Wang">Chooying Wang</name></author><author><name sortKey="Feng, Jing" sort="Feng, Jing" uniqKey="Feng J" first="Jing" last="Feng">Jing Feng</name></author><author><name sortKey="Duan, Jinzhu" sort="Duan, Jinzhu" uniqKey="Duan J" first="Jinzhu" last="Duan">Jinzhu Duan</name></author><author><name sortKey="Xie, Sishen" sort="Xie, Sishen" uniqKey="Xie S" first="Sishen" last="Xie">Sishen Xie</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15134191</idno><idno type="pmid">15134191</idno><idno type="wicri:Area/PubMed/Corpus">002E08</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002E08</idno><idno type="wicri:Area/PubMed/Curation">002E08</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002E08</idno><idno 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populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yan, Xiyun" sort="Yan, Xiyun" uniqKey="Yan X" first="Xiyun" last="Yan">Xiyun Yan</name></author><author><name sortKey="Cao, Wuchun" sort="Cao, Wuchun" uniqKey="Cao W" first="Wuchun" last="Cao">Wuchun Cao</name></author><author><name sortKey="Wang, Chooying" sort="Wang, Chooying" uniqKey="Wang C" first="Chooying" last="Wang">Chooying Wang</name></author><author><name sortKey="Feng, Jing" sort="Feng, Jing" uniqKey="Feng J" first="Jing" last="Feng">Jing Feng</name></author><author><name sortKey="Duan, Jinzhu" sort="Duan, Jinzhu" uniqKey="Duan J" first="Jinzhu" last="Duan">Jinzhu Duan</name></author><author><name sortKey="Xie, Sishen" sort="Xie, Sishen" uniqKey="Xie S" first="Sishen" last="Xie">Sishen Xie</name></author></analytic><series><title level="j">Antiviral therapy</title><idno type="ISSN">1359-6535</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans</term><term>Microscopy, Electron, Scanning (instrumentation)</term><term>Microscopy, Electron, Scanning (methods)</term><term>SARS Virus (ultrastructure)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Virion (ultrastructure)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Humains</term><term>Microscopie électronique à balayage ()</term><term>Microscopie électronique à balayage (instrumentation)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Virion (ultrastructure)</term><term>Virus du SRAS (ultrastructure)</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Microscopy, Electron, Scanning</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microscopy, Electron, Scanning</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>SARS Virus</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Humains</term><term>Microscopie électronique à balayage</term><term>Virion</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel coronavirus, SARS-CoV, has been confirmed to be the aetiological agent of SARS. Transmission electron microscope (TEM) images played an important role in initial identification of the pathogen. In order to obtain greater morphological detail of SARS-CoV than could be obtained by TEM, we used ultra-high resolution scanning electron microscopy (SEM) to image the virus particles. We show here the three-dimensional appearance of SARS-CoV. Enhanced detail of the ultrastructure reveals the trimeric structure of the 10-20 nm spikes on the virion surface. These results contribute to characterization of the SARS agent and development of new antiviral strategies.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Cao, Wuchun" sort="Cao, Wuchun" uniqKey="Cao W" first="Wuchun" last="Cao">Wuchun Cao</name><name sortKey="Duan, Jinzhu" sort="Duan, Jinzhu" uniqKey="Duan J" first="Jinzhu" last="Duan">Jinzhu Duan</name><name sortKey="Feng, Jing" sort="Feng, Jing" uniqKey="Feng J" first="Jing" last="Feng">Jing Feng</name><name sortKey="Wang, Chooying" sort="Wang, Chooying" uniqKey="Wang C" first="Chooying" last="Wang">Chooying Wang</name><name sortKey="Xie, Sishen" sort="Xie, Sishen" uniqKey="Xie S" first="Sishen" last="Xie">Sishen Xie</name><name sortKey="Yan, Xiyun" sort="Yan, Xiyun" uniqKey="Yan X" first="Xiyun" last="Yan">Xiyun Yan</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Lin, Yun" sort="Lin, Yun" uniqKey="Lin Y" first="Yun" last="Lin">Yun Lin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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